enzyme cox2  (BPS Bioscience)

Journal: Antioxidants

Article Title: Raspberry Ketones Attenuate Cyclophosphamide-Induced Pulmonary Toxicity in Mice through Inhibition of Oxidative Stress and NF-ΚB Pathway

doi: 10.3390/antiox9111168

Figure Lengend Snippet: Western blot analysis of NF-KB, iNOS, and COX-2 expression in the lung tissues of male mice treated with RKs against CP. ( A ) The lung tissues from different experimental groups were lysed and the proteins separated by SDS–PAGE and western blotted using antisera against COX-2, iNOS, NF-KB and β-actin was used as a loading control. The bar graphs show quantitative relative levels of ( B ) COX-2 ( C ) iNOS, and, ( D ) NF-KB protein expression for Control, CP (150 mg/kg, i.p.), CP+RKs with different doses (25, 50, 100, and 200 mg/kg/p.o.). All values are presented as means ± SE ( n = 4–6). Superscript symbols indicate a significant difference at p ≤ 0.05 using one- way ANOVA followed by the Bonferroni s test for multiple comparisons ( n = 3). a Indicates significant differences from +control (Control) mice, b Indicates significant differences from CP, c Indicates significant differences from RKs (25 mg/kg/p.o.).

Article Snippet: Antibodies against NF-KB (Cat# 8242S), cyclooxaganse-2 (COX-2) enzyme (Cat# 12282), and inducible nitric oxide synthase (iNOS) (Cat# 13120) were purchased from Cell Signaling Technology.

Techniques: Western Blot, Expressing, SDS Page, Control